Thymosin Alpha-1

Research overview

Thymosin Alpha-1 is a 28-residue N-acetylated peptide, Ac-SDAAVDTSSEITTKDLKEKKEVVEEAEN, first described in thymic extracts and also written as Tα1. It is studied for T-cell signalling and for innate and adaptive immune regulation in cell and preclinical models. Supplied at 5mg as a lyophilised powder for in-vitro laboratory research only.

Thymosin alpha-1 is processed from a larger precursor protein, prothymosin alpha. In the literature it is reported to act on antigen-presenting cells, with signalling through Toll-like receptors such as TLR2 and TLR9 and onward through NF-kappaB and MAPK pathways. Reported outcomes in cell models include dendritic cell maturation, changes in cytokine release and effects on T-cell differentiation markers. Findings differ between systems, and the receptor interactions are still being characterised.

Researchers use it in dendritic cell and T-cell culture assays, flow cytometry panels for activation markers, cytokine multiplex assays and reporter cell lines for Toll-like receptor signalling. Its N-terminal acetylation also makes it a useful model in peptide mass spectrometry work. It is supplied as a lyophilised powder in a 5mg vial.

Research areas

• Toll-like receptor signalling in dendritic cells
• T-cell differentiation and activation markers
• Cytokine release profiling
• Prothymosin alpha processing and biology
• N-acetylated peptide mass spectrometry

Specifications

CAS number

62304-98-7

Molecular formula

C129H215N33O55

Molecular weight

~3108.32 g/mol

Sequence

Ac-SDAAVDTSSEITTKDLKEKKEVVEEAEN (28-mer, N-acetylated)

Form

lyophilised powder

Purity

≥99% (HPLC)

Also known as

Thymosin Alpha-1, Tα1, Thymosin α1

Supplied as

5mg per vial, lyophilised powder

Storage and handling

Store the lyophilised powder in a freezer, protected from moisture and light, and let the vial reach room temperature before opening. Reconstitute with an appropriate laboratory diluent and keep reconstituted material cold.

Certificates of analysis

Each lot of Thymosin Alpha-1 is released with a certificate recording identity and purity. Enter the lot number printed on your vial on the lab testing page to view it.

Questions