ARA-290

Research overview

ARA-290 is an eleven-residue peptide derived from the helix B surface of erythropoietin, with an N-terminal pyroglutamate (pGlu-EQLERALNSS). It is studied in vitro and in preclinical models for tissue-protective receptor signalling that does not involve the red-cell pathway. Supplied at 10mg as a lyophilised powder for in-vitro laboratory research only.

Erythropoietin acts through two receptor forms. The homodimeric EPO receptor drives red-cell production, while a heteromer of the EPO receptor and the common beta chain (CD131) is described as an innate repair receptor linked to tissue protection. ARA-290 was designed from the part of erythropoietin thought to contact the second form, and in the literature it is reported not to bind the homodimer. Studies describe cytoprotective and anti-inflammatory signalling in cell and preclinical models.

Researchers use it in receptor-binding and co-expression studies with EPOR and CD131, in cell survival assays under stress, and in cultured neuronal, endothelial and immune cell models. Comparison with full-length erythropoietin helps separate tissue-protective from erythropoietic signalling. It is supplied as a lyophilised powder in a 10mg vial, also listed as HBSP.

Research areas

• Innate repair receptor (EPOR and CD131) signalling
• Cytoprotection under cell-stress conditions
• Neuronal and endothelial culture models
• Separating tissue-protective from erythropoietic pathways
• Short peptide design from erythropoietin helix B

Specifications

CAS number

1208243-50-8

Molecular formula

C51H84N16O21

Molecular weight

1257.3

Sequence

pGlu-Glu-Gln-Leu-Glu-Arg-Ala-Leu-Asn-Ser-Ser ([pE]EQLERALNSS; N-terminal pyroglutamate)

Form

lyophilised powder

Purity

≥99% (HPLC)

Also known as

ARA-290, Helix B Surface Peptide (HBSP), Erythropoietin-derived peptide

Supplied as

10mg per vial, lyophilised powder

Storage and handling

Store the lyophilised powder cold, dry and protected from light, and let the vial reach room temperature before opening. Reconstitute with an appropriate laboratory diluent and keep reconstituted material cold.

Certificates of analysis

Each lot of ARA-290 is released with a certificate recording identity and purity. Enter the lot number printed on your vial on the lab testing page to view it.

Questions