SNAP-8

Research overview

SNAP-8, or Acetyl Octapeptide-3, is an acetylated octapeptide, Ac-Glu-Glu-Met-Gln-Arg-Arg-Ala-Asp-NH2, that mimics part of SNAP-25. It is studied for SNARE-complex and neurotransmitter-release mechanisms in cell-free and cell-based systems, where it is compared with the native protein region it resembles. Supplied at 10mg as a lyophilised powder for in-vitro laboratory research only.

SNAP-25 is one of the three SNARE proteins, together with syntaxin and VAMP, whose assembly drives fusion of vesicles with the plasma membrane and so the release of neurotransmitter. SNAP-8 is described as an analogue of the N-terminal region of SNAP-25 that may compete with it for a place in the SNARE complex and so destabilise its formation. Evidence for this mechanism in cell-free and cell-based systems exists, but much of the published data comes from cosmetic-science and supplier studies, and the strength of the effect is debated.

Laboratory work includes cell-free SNARE assembly assays by fluorescence or gel shift, vesicle fusion and release assays in cultured neurosecretory cells such as PC12, and comparison with longer SNAP-25-derived peptides. Peptide stability in media is followed by HPLC, and control peptides with scrambled sequences are used to show that any effect depends on the specific residues. It is supplied as a lyophilised powder in a 10mg vial.

Research areas

• SNARE complex assembly assays
• Vesicle fusion and release in PC12 cells
• SNAP-25 mimetic peptide design
• Comparison with related acetylated peptides
• Peptide stability by HPLC

Specifications

CAS number

868844-74-0

Molecular formula

C41H70N16O16S

Molecular weight

1075.16 g/mol

Sequence

Ac-Glu-Glu-Met-Gln-Arg-Arg-Ala-Asp-NH2 (Ac-EEMQRRAD-NH2)

Form

lyophilised powder

Purity

≥99% (HPLC)

Also known as

SNAP-8, Acetyl Octapeptide-3, SNAP-25 analogue peptide

Supplied as

10mg per vial, lyophilised powder

Storage and handling

Store the lyophilised powder cold, dry and protected from light, and let the vial reach room temperature before opening. Reconstitute with an appropriate laboratory diluent and keep reconstituted material cold.

Certificates of analysis

Each lot of SNAP-8 is released with a certificate recording identity and purity. Enter the lot number printed on your vial on the lab testing page to view it.

Questions