PTD-DBM

Research overview

PTD-DBM is a 25-residue cell-penetrating peptide that combines an octa-arginine protein transduction domain, a GGGG linker and a Dishevelled-binding motif. It is built to interrupt a CXXC5-Dishevelled contact and is used in Wnt/beta-catenin signalling assays. Supplied at 5mg as a lyophilised powder for in-vitro laboratory research only.

CXXC5 is a negative regulator of Wnt/beta-catenin signalling. It binds Dishevelled (Dvl) through its PDZ domain and so dampens the pathway. The Dishevelled-binding motif in this peptide mimics the CXXC5 region that contacts Dvl, and the arginine-rich domain carries it across cell membranes. Competing for that contact is reported to release the brake on Wnt signalling, raising beta-catenin levels and TCF/LEF reporter activity in cultured cells.

Typical assays use TOPflash or similar TCF/LEF luciferase reporters, western blotting for beta-catenin and Dvl, pull-down and proximity assays for the CXXC5-Dvl interaction, and cultured dermal papilla or fibroblast cells. Controls with scrambled motifs or the transduction domain alone are standard. Cell-penetrating peptides can stick to plastic, so low-binding tubes are often used.

Research areas

• Wnt/beta-catenin reporter assays
• CXXC5-Dishevelled interaction
• Cell-penetrating peptide uptake
• Dermal papilla and fibroblast cultures
• Scrambled-motif and transduction-domain controls

Specifications

Molecular formula

C124H225N61O28S2

Molecular weight

~3082.62 g/mol (supplier analytical data)

Sequence

RRRRRRRRGGGGRKTGHQICKFRKC (octa-arginine PTD + GGGG linker + Dishevelled-binding motif, 25-mer)

Form

lyophilised powder

Purity

≥99% (HPLC)

Also known as

PTD-DBM; Protein Transduction Domain-Dishevelled Binding Motif peptide; CXXC5-Dishevelled interaction inhibitor

Supplied as

5mg per vial, lyophilised powder

Storage and handling

Store the lyophilised powder in a freezer, protected from moisture and light, and let the vial reach room temperature before opening. The peptide is highly charged and can stick to plastic, so use low-binding tubes after reconstitution.

Certificates of analysis

Each lot of PTD-DBM is released with a certificate recording identity and purity. Enter the lot number printed on your vial on the lab testing page to view it.

Questions