PE-22-28

Research overview

PE-22-28 is a seven-residue peptide, Gly-Val-Ser-Trp-Gly-Leu-Arg (GVSWGLR), taken from the sortilin propeptide and listed as a spadin-derived peptide. It is described as blocking the TREK-1 potassium channel and is used in ion-channel electrophysiology, including patch-clamp work on transfected cells and cultured neurons. Supplied at 5mg as a lyophilised powder for in-vitro laboratory research only.

TREK-1 is a two-pore-domain potassium channel that helps set resting membrane potential and responds to stretch, temperature, lipids and pH. The sortilin propeptide fragment spadin was reported to block TREK-1, and PE-22-28 is a shorter derivative described as keeping that blocking activity while being easier to make. In rodent neuron and cell-line preparations, TREK-1 blockade is reported to alter neuronal firing, which has made the channel a topic in neuropharmacology research.

Typical work uses patch-clamp recordings in HEK293 or COS-7 cells expressing TREK-1, with the peptide applied to the bath, and in cultured cortical or raphe neurons. Selectivity checks against other potassium channels, such as TREK-2 and TRAAK, are standard. Binding and stability studies by HPLC also feature. It is supplied as a lyophilised powder in a 5mg vial.

Research areas

• TREK-1 potassium channel blockade
• Patch-clamp recordings in transfected cells
• Firing properties of cultured cortical or raphe neurons
• Selectivity against other two-pore-domain channels
• Sortilin propeptide fragment pharmacology

Specifications

CAS number

1801959-12-5

Molecular formula

C35H55N11O9

Molecular weight

~773.89 g/mol

Sequence

Gly-Val-Ser-Trp-Gly-Leu-Arg

One-letter code

GVSWGLR

Form

lyophilised powder

Purity

≥99% (HPLC)

Also known as

PE-22-28, PE 22-28, spadin-derived peptide, sortilin propeptide (22-28) fragment

Supplied as

5mg per vial, lyophilised powder

Storage and handling

Store the lyophilised powder in a freezer, protected from moisture and light, and let the vial reach room temperature before opening. Reconstitute with an appropriate laboratory diluent and keep reconstituted material cold.

Certificates of analysis

Each lot of PE-22-28 is released with a certificate recording identity and purity. Enter the lot number printed on your vial on the lab testing page to view it.

Questions