P-21 (PO21)

Research overview

P-21 (PO21), also written P021, is a small synthetic peptide studied for neurogenesis and for the proliferation of neural progenitor cells, particularly in hippocampal models. It is described in different sources as ACTH-derived and as a mimetic of ciliary neurotrophic factor (CNTF). Supplied at 5mg as a lyophilised powder for in-vitro laboratory research only.

CNTF is a neurotrophic cytokine that supports neuron survival and neural progenitor activity. Short peptides modelled on its active region have been designed to keep neurotrophic signalling while being easier to handle than the full protein. Reported work in rodent hippocampal models and neural progenitor cultures describes changes in BDNF expression and in markers of progenitor proliferation. Because sources disagree on the origin and structure of P-21 (PO21), the analytical data supplied with each batch should be relied on rather than the name alone.

Laboratory uses include neurosphere and progenitor proliferation assays, immunostaining for neuronal differentiation markers, BDNF and signalling pathway analysis by qPCR and western blot, and primary hippocampal neuron cultures. Stability of the small peptide in culture media is usually measured alongside. The compound is supplied as a lyophilised powder in a 5mg vial.

Research areas

• Neural progenitor proliferation in neurosphere cultures
• Hippocampal neurogenesis models
• BDNF and neurotrophic pathway expression
• CNTF-mimetic peptide design
• Stability of small peptides in culture media

Specifications

CAS number

1246751-68-7

Molecular formula

C27H42N6O8

Molecular weight

~578.7 g/mol

Form

lyophilised powder

Purity

≥99% (HPLC)

Also known as

P-21, P021, CNTF mimetic peptide

Supplied as

5mg per vial, lyophilised powder

Storage and handling

Store the lyophilised powder in a freezer, protected from moisture and light, and let the vial reach room temperature before opening. Reconstitute with an appropriate laboratory diluent and keep reconstituted material cold.

Certificates of analysis

Each lot of P-21 (PO21) is released with a certificate recording identity and purity. Enter the lot number printed on your vial on the lab testing page to view it.

Questions