Kisspeptin-10

Research overview

Kisspeptin-10 is the active ten-residue fragment of kisspeptin, with the sequence YNWNSFGLRF and a C-terminal amide, also listed as a KiSS-1-derived decapeptide. It is used to study KISS1 receptor signalling and the hypothalamic-pituitary-gonadal axis. Supplied at 5mg as a lyophilised powder for in-vitro laboratory research only.

Kisspeptins are products of the KISS1 gene and act at the KISS1 receptor, a G protein-coupled receptor also known as GPR54. The C-terminal ten residues are reported to be enough for receptor binding and activation. Signalling runs mainly through Gq, raising intracellular calcium and activating protein kinase C. In preclinical models, kisspeptin signalling is described as a central regulator of gonadotropin-releasing hormone neurons, which makes the peptide a reference tool for the hypothalamic-pituitary-gonadal axis.

Typical assays include calcium mobilisation and inositol phosphate accumulation in KISS1R-expressing cells, receptor-binding competition studies, and electrophysiology on hypothalamic neurons in rodent brain slices. Comparisons with longer kisspeptin forms help map which regions matter for potency and stability, and receptor desensitisation after repeated exposure is a further topic. It is supplied as a lyophilised powder in a 5mg vial.

Research areas

• KISS1 receptor (GPR54) binding and Gq signalling
• Calcium mobilisation in receptor-expressing cells
• GnRH neuron electrophysiology in brain slices
• Hypothalamic-pituitary-gonadal axis models
• Kisspeptin fragment structure-activity work

Specifications

CAS number

374675-21-5

Molecular formula

C63H83N17O14

Molecular weight

~1302.4 g/mol

Sequence

Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH2 (YNWNSFGLRF, C-terminal amide)

Form

lyophilised powder

Purity

≥99% (HPLC)

Also known as

Kisspeptin-10, Metastin (10-10), KiSS-1-derived decapeptide, KISS1 receptor ligand fragment

Supplied as

5mg per vial, lyophilised powder

Storage and handling

Store the lyophilised powder in a freezer, protected from moisture and light, and let the vial reach room temperature before opening. Reconstitute with an appropriate laboratory diluent and keep reconstituted material cold.

Certificates of analysis

Each lot of Kisspeptin-10 is released with a certificate recording identity and purity. Enter the lot number printed on your vial on the lab testing page to view it.

Questions