MGF

Research overview

MGF, or mechano growth factor, is a 24-residue synthetic peptide modelled on the E domain of an IGF-1 splice variant, also called IGF-1Ec. It is studied in vitro and in preclinical models of mechanotransduction in myogenic cells and growth-factor signalling. Supplied at 2mg as a lyophilised powder for in-vitro laboratory research only.

Splicing of the IGF-1 gene produces several isoforms. The Ec variant, named MGF in rodents, was reported to be expressed in mechanically stressed and damaged tissue, and its E-domain peptide has been proposed to act separately from mature IGF-1. In myoblast cultures the peptide has been reported to promote proliferation and delay differentiation. Whether it works through the IGF-1 receptor or another route is disputed, and not every group has reproduced the effects.

Researchers use the peptide in myoblast and satellite-cell culture assays that measure proliferation, migration and differentiation markers, in receptor phosphorylation studies, and in comparisons against mature IGF-1 and PEGylated forms. Because short peptides are cleared quickly in culture, stability profiling by HPLC is also common. It is supplied as a lyophilised powder.

Research areas

• Mechanotransduction in myogenic cells
• IGF-1 splice-variant E-domain biology
• Myoblast and satellite-cell proliferation assays
• Receptor phosphorylation and signalling pathways
• Peptide stability and degradation profiling

Specifications

CAS number

12020-86-9

Molecular formula

C121H200N42O39

Molecular weight

2867.1

Sequence

YQPPSTNKNTKSQRRKGSTFEERK (Tyr-Gln-Pro-Pro-Ser-Thr-Asn-Lys-Asn-Thr-Lys-Ser-Gln-Arg-Arg-Lys-Gly-Ser-Thr-Phe-Glu-Glu-Arg-Lys)

Form

lyophilised powder

Purity

≥99% (HPLC)

Also known as

MGF, Mechano Growth Factor, IGF-1Ec, IGF-1 E-domain peptide, IGF-1 splice variant-derived peptide

Supplied as

2mg per vial, lyophilised powder

Storage and handling

Store the lyophilised powder in a freezer, protected from moisture and light, and let the vial reach room temperature before opening. Reconstitute with an appropriate laboratory diluent and keep reconstituted material cold.

Certificates of analysis

Each lot of MGF is released with a certificate recording identity and purity. Enter the lot number printed on your vial on the lab testing page to view it.

Questions