≥99% HPLCBatch testedIdentity by MS, purity by HPLC
Lot numberedCertificate filed per lot
Cold stored2 to 8 °C until dispatch
PNC-27
5mg lyophilised powderCAS 1159861-00-3
A p53-derived peptide used to study p53-related signalling and peptide-induced membrane disruption.
£34.90per vial, VAT included
In stock, dispatched same day before 16:00
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- Batch certificate in the COA Database
Sold for research purposes only. For in-vitro laboratory research. Not a medicine and not for human or animal consumption. Supplied to qualified individuals aged 18 and over; you confirm this at checkout.
Research overview
PNC-27 is a chimeric peptide, listed as a p53-derived and HDM-2-binding peptide, that joins a p53 sequence to a membrane-associating leader. It is used to study p53-related signalling and peptide-induced membrane disruption in cultured cells. Supplied at 5mg as a lyophilised powder for in-vitro laboratory research only.
The p53 region in this peptide is the part that binds HDM-2, also called MDM2. The attached leader sequence is described as helping the peptide enter or sit in cell membranes. In the literature, PNC-27 is reported to form pores in the membranes of some transformed cell lines by binding HDM-2 present at the cell surface, causing lysis, while non-transformed lines were reported to be spared. This model of membrane-surface HDM-2 is contested, and independent replication has been limited.
Laboratory work includes cell viability and lactate dehydrogenase release assays to detect membrane lysis, imaging of HDM-2 localisation, co-culture of transformed and non-transformed lines, and peptide-lipid interaction studies with model membranes. Control peptides with scrambled or leader-only sequences are standard. It is supplied as a lyophilised powder in a 5mg vial.
Research areas
- HDM-2 binding and p53-derived peptide design
- Membrane disruption and LDH release assays
- Cell-surface HDM-2 localisation by imaging
- Transformed versus non-transformed cell line comparisons
- Peptide-lipid interaction in model membranes
Specifications
- CAS number
- 1159861-00-3
- Molecular formula
- C188H293N53O44S
- Molecular weight
- 4031.72 g/mol
- Form
- lyophilised powder
- Purity
- ≥99% (HPLC)
- Also known as
- PNC-27, p53-derived peptide, HDM-2-binding peptide
- Supplied as
- 5mg per vial, lyophilised powder
Storage and handling
Store the lyophilised powder in a freezer, protected from moisture and light, and let the vial reach room temperature before opening. Reconstitute with an appropriate laboratory diluent, aliquot, and keep reconstituted material cold.
Certificates of analysis
Each lot of PNC-27 is released with a certificate recording identity and purity. Enter the lot number printed on your vial on the lab testing page to view it.
Questions
What are the two parts of PNC-27?
One part is a p53-derived sequence that binds HDM-2. The other is a leader sequence described as helping the peptide associate with cell membranes. Control peptides lacking either part are used to test which element is responsible for an effect.
How is PNC-27 supplied and stored?
It is supplied as a lyophilised powder in a 5mg vial. Keep it cold, dry and away from light, and reconstitute with a suitable laboratory diluent only when the material is needed.
Is PNC-27 a medicine?
No. It is supplied strictly for in-vitro laboratory research. It is not a medicine, food or supplement, and it is not for human or animal use. Buyers must be aged 18 or over.
