MOTS-c

Research overview

MOTS-c is a 16-residue peptide encoded in the mitochondrial genome, within the 12S rRNA gene, with the sequence MRWQEMGYIFYPRKLR. It is studied in metabolic regulation and cellular energy homeostasis in cell and preclinical models. Supplied at 10mg as a lyophilised powder for in-vitro laboratory research only.

MOTS-c belongs to a small group of mitochondrial-derived peptides. Reported work in cell and preclinical models links it to folate and one-carbon metabolism, to accumulation of AICAR and to activation of the energy sensor AMPK. Under metabolic stress it has also been reported to move into the nucleus and influence gene expression, including genes with antioxidant response elements. These pathways are still under active study, and many findings come from a limited number of groups.

Laboratory uses include AMPK phosphorylation assays by western blot, glucose uptake and mitochondrial respiration measurements in myotube and hepatocyte cultures, nuclear translocation imaging, and gene expression profiling under stress conditions. Because it is a mitochondrial-derived sequence, it is also a useful model for studying how small open reading frames are annotated. It is supplied as a lyophilised powder in a 10mg vial.

Research areas

• AMPK activation in cultured myotubes and hepatocytes
• Folate and one-carbon metabolism
• Mitochondrial respiration and glucose uptake assays
• Nuclear translocation under cell stress
• Mitochondrial-derived peptide biology

Specifications

CAS number

1627580-64-6

Molecular formula

C101H152N28O22S2

Molecular weight

~2174.64 g/mol

Sequence

MRWQEMGYIFYPRKLR (16-mer; Met-Arg-Trp-Gln-Glu-Met-Gly-Tyr-Ile-Phe-Tyr-Pro-Arg-Lys-Leu-Arg)

Form

lyophilised powder

Purity

≥99% (HPLC)

Also known as

MOTS-c, Mitochondrial ORF of the Twelve S rRNA-c

Supplied as

10mg per vial, lyophilised powder

Storage and handling

Store the lyophilised powder in a freezer, protected from moisture and light, and let the vial reach room temperature before opening. Reconstitute with an appropriate laboratory diluent and keep reconstituted material cold.

Certificates of analysis

Each lot of MOTS-c is released with a certificate recording identity and purity. Enter the lot number printed on your vial on the lab testing page to view it.

Questions